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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Digoxigenin NHS ester/3500/1 mg
产品编号:3500
市  场 价:¥42060.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$2103.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Digoxigenin NHS ester/3500/1 mg
商品介绍
Overview
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Ex/Em(nm)None/None
MW658.79
CAS#129273-26-3
SolventDMSO
StorageF/D
CategoryProtein-BasedTags
TagAntibodies
Related
Digoxigenin(DIG)isahapten,asmallmoleculewithhighantigenicity,whichisusedinmanyBIOLOGicaldetectionssimilarlytootherpopularhaptenssuchas2,4-Dinitrophenol(DNP)andbiotin.Typically,digoxigeninisconjugatedtoamacrobiomolecule(e.g.,proteins,nucleicacids,etc)tobedetected.Anti-digoxigeninantibodiesarelabeledwithfluorescentdyesorenzymes,directlyorsecondarily,forvisualizationanddetection.DigoxigeninNHSesterisanactivatedesterwhichreADIlyreactswithaminogroupsundermildconditions,attachingthedigoxigeninmoietytoproteinsoramino-substitutedoligonucleotides.Digoxigenin-labledbiomoleculesareusedforbiologicaldetectionsincombinationwithanti-digoxigeninantibodiesofhighaffinityandspecificity.
References&Citations
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1.   Baumgart,E.;Schad,A.;Volkl,A.;Fahimi,H.D.,DetectionofmRNAsencodingperoxisomalproteinsbynon-radioactiveinsituhybridizationwithdigoxigenin-labelledcRNAs.HistochemCellBiol1997,108(4-5),371-9.

2.   McCreery,T.,Digoxigeninlabeling.MolBiotechnol1997,7(2),121-4.

3.   Chevalier,J.;Yi,J.;Michel,O.;Tang,X.M.,Biotinanddigoxigeninaslabelsforlightandelectronmicroscopyinsituhybridizationprobes:wheredowestand?JHistochemCytochem1997,45(4),481-91.

4.   Kubota,S.;Takabe,Y.,[Detectionofgeneexpressionusingdigoxigenin-labeledprobes].TanpakushitsuKakusanKoso1996,41(5),486-90.

5.   Holtke,H.J.;Ankenbauer,W.;Muhlegger,K.;Rein,R.;Sagner,G.;SeIBL,R.;Walter,T.,Thedigoxigenin(DIG)systemfornon-radioactivelabellinganddetectionofnucleicacids--anoverview.CellMolBiol(Noisy-le-grand)1995,41(7),883-905.

6.   Haselbeck,A.;Hosel,W.,Immunologicaldetectionofglycoproteinsonblotsbasedonlabelingwithdigoxigenin.MethodsMolBiol1993,14,161-73.

7.   Kessler,C.,Thedigoxigenin:anti-digoxigenin(DIG)technology--asurveyontheconceptandrealizationofanovelbioanalyticalindicatorsystem.MolCellProbes1991,5(3),161-205.

8.   Graves,L.M.;Swaminathan,B.;Reeves,M.W.;Wenger,J.,RibosomalDNAfingerprintingofListeriamonocytogenesusingadigoxigenin-labeledDNAprobe.EurJEpidemiol1991,7(1),77-82.


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