请使用支持JavaScript的浏览器!
主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/ReadiLink™ Rapid FITC Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per R
产品编号:1299
市  场 价:¥42060.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$2103.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/ReadiLink™ Rapid FITC Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per R
商品介绍
Overview
PrinterFriendlyVersion

Ex/Em(nm)492/516
MWN/A
CAS#N/A
SolventDMSO
StorageF/D/L
CategorySuperiorLabelingDyes
iFluor™DyesandKits
RelatedGeneralproteins
LabelingviaAminoGroups
BiochemicalAssays
FITCisstillthemostpopularfluorescentlabelingdyeforpreparinggreenfluorescentbioconjugates.However,mostofcommercialFITClabelingkitsrequireintensivehands-ontime.ThisFITCReadiLink™labelingkitessentiallyonlyrequires2simplemixingstepswithoutacolumnpurificationneeded.Thekitprovidesalltheessentialcomponentsforlabeling~2x50ugantibody.EachofthetwovialsofFITCdyeprovidedinthekitisoptimizedforlabeling~50µgantibody.ThisFITCproteinlabelingkitprovidesaconvenientmethodtolabelmonoclonal,polyclonalantibodiesorotherproteins(>10kDa).
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
Sorry,yourbrowserdoesnotsupportinlineSVG.Sorry,yourbrowserdoesnotsupportinlineSVG.
Movemouseovergridtodisplaywavelength&intensityvalues.

300
400
500
600
700
800
900
Wavelength(nm)


Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.
  1. Prepareproteinsolution(SolutionA):
    Forlabeling50ugprotein(assumingthetargetproteinconcentrationis1mg/mL),mix5μL(10%ofthetotalreactionvolume)ofReactionBuffer(ComponentB)with50uLofthetargetproteinsolution.
    Note1:Ifyouhaveadifferenceproteinconcentration,adjusttheproteinvolumeaccordinglytomake~50µgproteinavailableforyourlabelingreaction.
    Note2:Forlabeling100ugprotein(assumingthetargetproteinconcentrationis1mg/mL),mix10uL(10%ofthetotalreactionvolume)ofReactionBuffer(ComponentB)with100uLofthetargetproteinsolution.
    Note3:Theproteinshouldbedissolvedin1Xphosphatebufferedsaline(PBS),pH7.2-7.4;Iftheproteinisdissolvedinglycinebuffer,itmustbedialyzedagainst1XPBS,pH7.2-7.4,oruseAmiconUltra-0.5,Ultracel-10Membrane,10kDa(cat#UFC501008fromMillipore)toremovefreeaminesorammoniumsalts(suchasammoniumsulfateandammoniumacetate)thatarewidelyusedforproteinprecipitation.
    Note4:ImpureantibodiesorantibodiesstABIlizedwithbovineserumalbumin(BSA)orgelatinwillnotbelabeledwell.
    Note5:Theconjugationefficiencyissignificantlyreducediftheproteinconcentrationislessthan1mg/mL.Foroptimallabelingefficiencythefinalproteinconcentrationrangeof1-2mg/mLisrecommended.

  2. Runconjugationreaction::
    1. Addtheproteinsolution(SolutionA)toONEvialoflabelingdye(ComponentA),andmixthemwellbyrepeatedlypipettingforafewtimesorvortexthevialforafewseconds.
      Note:Usebothvials(ComponentA)oflabelingdyetolabel100ugproteinbydividingthe100ugproteininto2x50ugproteinandreactingeach50ugproteinwithonevialoflabelingdye.Combinetwovialsforthenextstep.
    2. Keeptheconjugationreactionmixtureatroomtemperaturefor30-60minutes.
      Note:Theconjugationreactionmixturecanberotatedorshakenforlongertimeifdesired.

  3. StopConjugationreaction:
    1. Add5uL(for50ugprotein)or10uL(for100ugprotein)whichis10%ofthetotalreactionvolumeofTQ-DyedQuenchBuffer(ComponentC)intotheconjugationreactionmixture(fromstep2.2),mixthemwell.
    2. Incubateatroomtemperaturefor10minutes.
    3. Thelabeledprotein(antibody)isnowreadytouse.
References&Citations
CitationExplorer

Roleofreactiveoxygenspecies-mediatedMAPKandNF-$kappa$Bactivationinpolygonatumcyrtonemalectin-inducedapoptosisandautophagyinhumanlungadenocarcinomaA549cells
Authors:TaoLiu,LeiWu,DiWang,HaiyangWang,JinwuChen,ChunlanYang,JinkuBao,ChuanfangWu
Journal:JournalofBiochemistry(2016):mvw040

Determinationofsodiumbenzoateinfoodproductsbyfluorescencepolarizationimmunoassay
Authors:LinlinRen,MengMeng,PengWang,ZhihuanXu,SergeiAEremin,JunhongZhao,YongmeiYin,RimoXi
Journal:Talanta(2014):136--143

ComplexescontainingcationicandanionicpH-sensitiveliposomes:comparativestudyoffactorsinfluencingplasmidDNAgenedeliverytotumors
Authors:YanChen,JiSun,YingLu,ChunTao,JingbinHuang,HeZhang,YuanYu,HaoZou,JingGao,YanqiangZhong
Journal:Internationaljournalofnanomedicine(2013):1573

SwimmingbehaviorofthemonotrichousbacteriumPseudomonasfluorescensSBW25
Authors:LiyanPing,JanBirkenbeil,ShamciMonajembashi
Journal:FEMSmicroBIOLOGyecology(2013):36--44


品牌介绍

AAT Bioquest常用产品报价单

产品名货号公司分类商城分类美金价
AAT Bioquest/Fluorescein, disodium salt *CAS 518-47-8*/2/100 mg2iFluor Rapid Tests酸碱缓冲液1088.00
AAT Bioquest/14-3-3 ζ (Ab-58) Antibody/8B0001/50 ug8B0001Signaling Intermediate Ab重组抗体2828.00
AAT Bioquest/Opioid Receptor (Phospho-Ser375) Antibody/8A0022/50 ug8A0022Phospho-Specific Ab受体2828.00
AAT Bioquest/iFluor™ 350 goat anti-mouse IgG (H+L)/16440/200 ug16440Fluorescent Anti-IgGs荧光染料1088.00
AAT Bioquest/trFluor™ Eu goat anti-mouse IgG (H+L)/16518/100 ug16518Fluorescent Anti-IgGs抗小鼠4278.00
AAT Bioquest/RPE-streptavidin conjugate/16900/100 ug16900Streptavidin Conjugates其他生物染料1378.00
AAT Bioquest/GCNT3 Antibody/8C14708/50 ug8C14708Signaling Intermediate Ab功能性抗体2828.00
AAT Bioquest/Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*/12518/1 plate12518Reporter Gene Enzymes其它检测试剂盒2103.00
AAT Bioquest/ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 &mi1100mFluor™ Dyes and Kits标记试剂盒2103.00
AAT Bioquest/Amplite™ Colorimetric Urea Quantitation Kit *Blue Color*/10058/200 Tests10058Diagnostic Molecules定量试剂盒2828.00
AAT Bioquest/Screen Quest™ CHO-Gqi Chimera Cell line/38101/Each38101cAMP GPCR Assays常规细胞株0.00
自营商城图标
厂家直采
全球直采 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔