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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Fluorescein biotin/3017/5 mg
产品编号:3017
市  场 价:¥27560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$1378.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Fluorescein biotin/3017/5 mg
商品介绍
Overview
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Ex/Em(nm)492/515
MW831.01
CAS#N/A
SolventDMSO
StorageF/D
CategoryBiotinandItsDerivatives
BiotinConjugates
Related
Thisbifunctionalbiotinderivativeisusedforspectrophotometricdeterminationofbiotinylationdegree.Theassayisbasedonthekineticanalysisoftheenhancementoffluorescenceofstreptavidin/fluoresceinbiotincomplexesinthepresenceofbiotin.Thefluorescenceenhancementoffluoresceinbiotinisproportionaltotheconcentrationofbiotin.Becausetheassayisamenableforuseinsmallvolumesof5-50uLorbead-basedassays,thedetectionlimitscanbeextendedtothefemtomolerange.Thedynamicaspectsallowtheassaytobeextendedtoabroaderrangeofapplicationsincludingitsuseasanindicatorofreagentmixinginlaminar-flowassayscarriedoutinmicrofluidicdevices.
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
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References&Citations
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1.   AslanFM,YuY,MohrSC,CantorCR.(2005)Engineeredsingle-chaindimericstreptavidinswithanunexpectedstrongpreferenceforbiotin-4-fluorescein.ProcNatlAcadSciUSA,102,8507.

2.   WuY,SimonsPC,LopezGP,SklarLA,BurandaT.(2005)Dynamicsoffluorescencedequenchingofostrich-quenchedfluoresceinbiotin:amultifunctionalquantitativeassayforbiotin.AnalBiochem,342,221.

3.   HumbertN,ZocchiA,WardTR.(2005)Electrophoreticbehaviorofstreptavidincomplexedtoabiotinylatedprobe:afunctionalscreeningassayforbiotin-bindingproteins.Electrophoresis,26,47.

4.   BalthasarS,MichaelisK,DinauerN,vonBriesenH,KreuterJ,LangerK.(2005)Preparationandcharacterisationofantibodymodifiedgelatinnanoparticlesasdrugcarriersystemforuptakeinlymphocytes.Biomaterials,26,2723.

5.   SchiestelT,BrunnerH,TovarGE.(2004)Controlledsurfacefunctionalizationofsilicananospheresbycovalentconjugationreactionsandpreparationofhighdensitystreptavidinnanoparticles.JNanosciNanotechnol,4,504.

6.   HuangF,WangG,ColemanT,LiN.(2003)Synthesisofadenosinederivativesastranscriptioninitiatorsandpreparationof5"fluorescein-andbiotin-labeledRNAthroughone-stepinvitrotranscription.Rna,9,1562.

7.   HoyaK,GutermanLR,MiskolcziL,HopkinsLN.(2001)Anovelintravasculardrugdeliverymethodusingendothelialbiotinylationandavidin-biotinbinding.DrugDeliv,8,215.

8.   WuMM,LlopisJ,AdamsS,McCafferyJM,KulomaaMS,MachenTE,MooreHP,TsienRY.(2000)OrganellepHstudiesusingtargetedavidinandfluorescein-biotin.ChemBiol,7,197.

9.   KadaG,KaiserK,FalkH,GruberHJ.(1999)Rapidestimationofavidinandstreptavidinbyfluorescencequenchingorfluorescencepolarization.BiochimBiophysActa,1427,44.

10.   KadaG,FalkH,GruberHJ.(1999)Accuratemeasurementofavidinandstreptavidinincrudebiofluidswithanew,optimizedbiotin-fluoresceinconjugate.BiochimBiophysActa,1427,33.


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