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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Biocytin C2 maleimide/3085/5 mg
产品编号:3085
市  场 价:¥56560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$2828.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Biocytin C2 maleimide/3085/5 mg
商品介绍
Overview
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Ex/Em(nm)None/None
MW494.61
CAS#N/A
SolventDMSO
StorageF/D
CategoryBiotinandItsDerivatives
Thiol-ReactiveBiotins
RelatedThiol-ReactiveProbes
BiochemicalAssays
BiocytinmaleimidereADIlyreactswiththiolmoietiesofbiopolymerstoformthioetherconjugatethatisquitestable.Thisbiocytinmaleimiderequiresmildconjugationconditions.Forexample,pHof5.5-8.5isusuallyoptimalformodifyingcysteineresidues,andairexposureofreactionsolutionshouldbeminimizedwheneverpossIBLetoavoidtheairoxidationofthiolsubstrates.Mostconjugationsaredoneatroomtemperature.However,eitherelevatedorreducedtemperaturemayberequiredforaparticularlabelingreaction.Reactionswiththisbiotinylationreagentshouldbeperformedinbuffersfreeofextraneousthiols(suchas6-mercaptoethanol,dithiothreitolandmercaptoethylamine).Proteinsorpeptidestobebiotinylatedbythiol-reactivereagentsmusthaveafreethiolgroup(SH)available.
References&Citations
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1.   XueHG,YangCY,ItoH.(2004)Theanterogradeandretrogradeaxonaltransportofbiotinylateddextranamineandbiocytininthenervoussystemofteleosts.BrainResBrainResProtoc,13,106.

2.   MorozovY,KhalilovI,Ben-AriY,RepresaA.(2002)Correlativefluorescenceandelectronmicroscopyofbiocytin-filledneuronswithapreservationofthepostsynapticultrastructure.JNeurosciMethods,117,81.

3.   BaurB,SuormalaT,BaumgartnerER.(2002)BiocytinandbiotinuptakeintoNB2aneuroblastomaandC6astrocytomacells.BrainRes,925,111.

4.   KumasakaK,MuratsuguM,FukuiT,KimuraM,TakagiY,HashizumeN.(2001)AnewquantitativeanalyticalmethodofserumbiotinidaseactivityusingbiocytinasasubstrateanditsclinicalsignificanceinJapan.ClinChimActa,306,71.

5.   ChangSL,LoTurcoJJ,NisenbaumLK.(2000)Invitrobiocytininjectionintoperinatalmousebrain:amethodfortracttracingindevelopingtissue.JNeurosciMethods,97,1.

6.   BaurB,BaumgartnerER.(2000)Biotinandbiocytinuptakeintoculturedprimarycalfbrainmicrovesselendothelialcellsoftheblood-brainbarrier.BrainRes,858,348.

7.   NakamotoY,SakaharaH,SagaT,YaoZ,ZhangM,SatoN,ZhaoS,AranoY,OnoM,SajiH,KonishiJ.(1998)Aradioiodinatedbiocytinderivativeforin-vivoapplications.NuclMedCommun,19,963.

8.   ErisirA,AokiC.(1998)Amethodofcombiningbiocytintract-tracingwithavidin-biotin-peroxidasecompleximmunocytochemistryforpre-embeddingelectronmicroscopiclabelinginneonataltissue.JNeurosciMethods,81,189.

9.   SunXJ,TolbertLP,HildebrandJG,MeinertzhagenIA.(1998)Arapidmethodforcombinedlaserscanningconfocalmicroscopicandelectronmicroscopicvisualizationofbiocytinorneurobiotin-labeledneurons.JHistochemCytochem,46,263.

10.   ScaliaF,GaloyanSM,EisnerS,HarrisE,SuW.(1997)Biotinylateddextranamineandbiocytinhydrochlorideareusefultracersforthestudyofretinalprojectionsinthefrog.JNeurosciMethods,76,167.


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