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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite]/6025/10x100 umoles
产品编号:6025
市  场 价:¥420500.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$21025.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite]/6025/10x100 umoles
商品介绍
Overview
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Ex/Em(nm)521/541
MW981.72
CAS#877049-90-6
SolventMeCN
StorageF/D/L
CategoryOligoSynthesis
DyePhosphoramidites
RelatedHydroxy-ReactiveProbes
BiochemicalAssays
Thereareseveralwaysoflabelinganoligonucleotidewithfluorescein.Thechoiceoflabelisdiversifiedfurther,dependingonthespectralrequirements.5"-fluorescein-CEphosphoramidite,derivedfromthesingleisomer6-carboxyfluorescein,5"-hexachlorofluorescein-CEphosphoramidite(HEX)and5"-tetrachlorofluorescein-CEphosphoramidite(TET)canallbeusedtoefficientlylabelanoligonucleotideatthe5"-end.Labelingthe3"-endofanoligowithfluoresceincanbeachievedusing3"-FluoresceinCPGs.Standardcleavageanddeprotectionwithammoniumhydroxideliberatesthefluorescein-labeledoligowhenusinganyofthesesupports.
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
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References&Citations
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1.   BrowneKA.(2005)Sequence-specific,self-reportinghairpininversionprobes.JAmChemSoc,127,1989.

2.   DobsonN,McDowellDG,FrenchDJ,BrownLJ,MellorJM,BrownT.(2003)SynthesisofHyBeaconsanddual-labelledprobescontaining2"-fluorescentgroupsforuseingeneticanalysis.ChemCommun(Camb),1234.

3.   HagmarP,BaileyM,TongG,HaralambidisJ,SawyerWH,DavidsonBE.(1995)Synthesisandcharacterisationoffluorescentoligonucleotides.Effectofinternallabellingonproteinrecognition.BiochimBiophysActa,1244,259.

4.   HakalaH,VirtaP,SaloH,LonnbergH.(1998)Simultaneousdetectionofseveraloligonucleotidesbytime-resolvedfluorometry:theuseofamixtureofcategorizedmicroparticlesinasandwichtypemixed-phasehybridizationassay.NucleicAcidsRes,26,5581.

5.   HillKW,Taunton-RigbyJ,CarterJD,KroppE,VagleK,PiekenW,McGeeDP,HusarGM,LeuckM,AnzianoDJ,SebestaDP.(2001)Diels--Alderbioconjugationofdiene-modifiedoligonucleotides.JOrgChem,66,5352.

6.   KimSJ,BangEK,KwonHJ,ShimJS,KimBH.(2004)Modifiedoligonucleotidescontaininglithocholicacidintheirbackbones:theirenhancedcellularuptakeandtheirmimickingofhairpinstructures.Chembiochem,5,1517.

7.   KutyavinIV,LokhovSG,AfoninaIA,DempcyR,GallAA,GornVV,LukhtanovE,MetcalfM,MillsA,ReedMW,SandersS,ShishkinaI,VermeulenNM.(2002)ReducedaggregationandimprovedspecificityofG-richoligodeoxyribonucleotidescontainingpyrazolo[3,4-d]pyrimidineguaninebases.NucleicAcidsRes,30,4952.

8.   LeeSP,CensulloML,KimHG,KnutsonJR,HanMK.(1995)CharacterizationofendonucleolyticactivityofHIV-1integraseusingafluorogenicsubstrate.AnalBiochem,227,295.

9.   MeyerKL,HannaMM.(1996)Synthesisandcharacterizationofanew5-thiol-protecteddeoxyuridinephosphoramiditeforsite-specificmodificationofDNA.BioconjugChem,7,401.

10.   NelsonPS,KentM,MuthiniS.(1992)Oligonucleotidelabelingmethods.3.Directlabelingofoligonucleotidesemployinganovel,non-nucleosidic,2-aminobutyl-1,3-propanediolbackbone.NucleicAcidsRes,20,6253.


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